Purpose:Hepatitis B virus(HBV) plays a crucial role in the progression of hepatocellular carcinoma(HCC).It is known that HBV-encoded X protein(HBx) canColforsin分子式 induce genetic alterations in some oncogenes and that SMAD4 is relevant for the development of some cancers,especially HBV-related HCC.Previously,it has been reported that HBx can promote SMAD4 protein expression in liver fibrosis and HCC but,as yet,its regulatory mechanism has not been fully elucidated.Here,we aimed to investigate the correBrain biomimicrylation between and regulatory mechanism behind HBx and SMAD4 in HCC.Methods:mRNA and protein expression of SMAD4 in HCC tissues was detected by qRT-PCR,Western blotting and IHC.CCK-8 and colony forming assays,as well as xenograft murine models were used to evaluate the effects of HBx and SMAD4 on the prolifhttps://www.selleck.cn/products/epz-6438.htmleration and tumorigenicity of HCC cells.Luciferase reporter,immunofluorescence,Co-IP and truncation assays were performed to assess the regulatory relationship between HBx and SMAD4.Results:We found that SMAD4 was highly …