Background:Obesity has become a major public health problem and ferroptosis is associated with obesity and metabolic diseases.The purpose of this study was Biomass exploitationto investigate whether β-carotene can reduce high-fat diet induced obesity by regulating lipid peroxidation associated with ferroptosis in liver. Method:Four-week-old male C57BL/6J mice were randomly divided into four groups and fed as follows:1)control group(10% of calories from fat,n=8),2)control group with β-carotene(n = 8),3)high-fat diet group(60% of calories from fat,n =8) and 4)high-fat diet with β-carotene(n =8).After 12 weeks’ feeding,the obese mouse model was established and then β-carotene(3mg/kg) was given intragastric administration. Result:1.After 8 weeks’ intervention,the weight,body fat and the expression of ferroptosis related proteins between control group and control group with β-carotene were no statistical difference.Compared with high-fat diet group,the weight,body fat,the area under curve of Oral Glucose Tolerance Test and Insulin Tolerance Test,serum contents of Fe,triglyceride,low-density lipoprotein cholesterol and total cholesterol in high-fat diet with β-carotene group were significantly decreased(P<0.05). 2.Hematoxylin-eosin staining showed that compared with high-fat diet group,the lipid droplet in thttps://www.selleck.cn/products/cobimetinib-gdc-0973-rg7420.htmlhe liver of high-fat diet with β-carotene group were significantly reduced. 3.Compared with control group,the protein expression of Ferritin light chain,Heme oxygenase-1,Cyclooxygenase-2,Acyl-CoA synthetase long chain family member 4,Ferritin heavy chain and Transferrin in high-fat diet group was significantly increased;Glutathione peroxidase 4 and Ferroportin1 was downregulated(P<0.05).Compared with high-fat diet group,those protein expression in highfat diet with β-carotene group was significantly down-regulated,Glutathione peroxidase 4 and Ferroportin1 was increased(P<0.05),which is consistent with the results of Immunohistochemistry. Conclusion:After 8 weeks of β-carotene intragastric administration,the weight,body fat,glycolipid metabolism and lipid peroxidation associated with ferroptosis of the liver in high-fat diet induced obese mice had been improved.We demonstrated that β-carotene alleviates high-fat diet induced obesity by improving hepatic lipid peroxidation induced by ferroptosis,which provided a perspective for further research on the mechanism of reducing obesity and related metabolic diseases by inhibiting feselleck LY2157299rroptosis related pathways.