The essential oils of Chrysanthemum morifolium Ramat were extracted by steam distillation and analyzed by Gas chromatography-mass spectrometry(GC–MS). The volatile oil was evaluated for antibacterial activity against five Gram-positive bacteria(Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, V. Streptococci, S. pneumoniae), three Gram-negative bacteria(Escherichia coli, Pseudomonas aeruginosa, Shigella flexneri) and two molds(Candida albicans, Cryptococcus neoforman). In vitro, cell proliferation inhibition action on human liver cancer HepG-2 was examined by 3-(4,5-dimethyl)-2,5-diphenyl-2H(MTT) method and cell apoptosis was observed using flow cytometry. In vivo,INCB018424试剂 S-180 tumor-bearing mice were used to research antitumor effect. A total of 59 compounds were identified and caryophyllene oxide was the most abundant volatile component(7.39%). The volatile oil showed promising antibacterial activity against 5 Gram-positive bacteria(S. aureus, S. epidermidis, B. subtilis, V. Streptococci, S. pneumoniae) and 2 molds(Candida albicans, Cryptococcus neoforman). The oils showed significant antitumor activity towards HepG-2 cells with IC50 valued 1.42 μL/mL and increased the apoptotic rates in a dose dependent manner. In addition, the oils inhibited tumor growth of S-180 tumor-bearing mice and the tumor suppressor rate of high dose reached 54.94%. Giving the oils improved the thymus/weight index of tuAntibiotic urine concentrationmor-bearing mice which means it increased immune function. All these findings suggest that the essential oil has antibacterial and antitumor activity. It is a candidate for exploKD025半抑制浓度ring new health foods, the food additive and drugs.